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primary human bmscs (hbmscs) scc034  (Millipore)

 
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    Structured Review

    Millipore primary human bmscs (hbmscs) scc034
    A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of <t>hBMSCs.</t> Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Primary Human Bmscs (Hbmscs) Scc034, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human bmscs (hbmscs) scc034/product/Millipore
    Average 90 stars, based on 1 article reviews
    primary human bmscs (hbmscs) scc034 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Matrix stiffness and stress relaxation regulate osteogenesis through histone demethylases KDM4B and KDM6B"

    Article Title: Matrix stiffness and stress relaxation regulate osteogenesis through histone demethylases KDM4B and KDM6B

    Journal: bioRxiv

    doi: 10.1101/2025.07.16.665229

    A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Staining, Gene Expression, Cell Culture



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    primary human bmscs (hbmscs) scc034  (Millipore)
    90
    Millipore primary human bmscs (hbmscs) scc034
    A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of <t>hBMSCs.</t> Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Primary Human Bmscs (Hbmscs) Scc034, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human bmscs (hbmscs) scc034/product/Millipore
    Average 90 stars, based on 1 article reviews
    primary human bmscs (hbmscs) scc034 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: bioRxiv

    Article Title: Matrix stiffness and stress relaxation regulate osteogenesis through histone demethylases KDM4B and KDM6B

    doi: 10.1101/2025.07.16.665229

    Figure Lengend Snippet: A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary human BMSCs (hBMSCs) were obtained from Millipore Sigma (SCC034) and cultured according to established protocols ( Huebsch et al ., 2010 ; Darnell et al ., 2017 ).

    Techniques: Staining, Gene Expression, Cell Culture